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. 2020 Aug 25;11:1076. doi: 10.3389/fphys.2020.01076

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Copyright © 2020 de Araújo, Costa-Pessoa, de Ponte and Oliveira-Souza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of NaOx treatment on total megalin protein expression. (A,B) Immunoblot analysis was performed on 50-μg protein aliquots resolved by 4% SDS-PAGE. The values are presented as the mean ± SE (n = 4/group) and β-actin was used as an internal control. Megalin staining in the proximal tubule (PT) (C) is indicated by the arrows. Images of megalin immunofluorescence were captured using a Zeiss LSM 510 confocal microscope equipped with a 63× objective. Statistical analysis of megalin immunofluorescence in both the control and treated groups was performed (D). CTL, control; NaOx, sodium oxalate; G, glomerulus. Bar = 50 μm.