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. 2020 Aug 25;8:974. doi: 10.3389/fbioe.2020.00974

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Copyright © 2020 Moriya, Takita, Matsumoto, Yamahata, Nishimukai, Miyazaki, Shimoi, Kawai and Yamada.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of alginate concentration, NaCl concentration, and pH on growth and P(3HB) production in Cobetia sp. IU180733JP01 (5-11-6-3) and Cobetia sp. IU190790JP01 (5-25-4-2). (A) Effect of alginate concentration on growth, P(3HB) production, and P(3HB) content of Cobetia sp. IU180733JP01 (5-11-6-3) in the MM medium containing 2% NaCl at 30°C, pH 5.0 for 3 days. (B) Effect of alginate concentration on growth, P(3HB) production, and P(3HB) content of Cobetia sp. IU190790JP01 (5-25-4-2) in the MM medium containing 2% NaCl at 30°C, pH 5.0, for 2 days. (C) Effects of NaCl concentration on growth, P(3HB) production, and P(3HB) content of Cobetia sp. IU180733JP01 (5-11-6-3) in the MM medium containing 3% (w/v) alginate at 30°C, pH 5.0 for 3 days. (D) Effect of NaCl concentration on growth, P(3HB) production, and P(3HB) content of Cobetia sp. IU190790JP01 (5-25-4-2) in the MM medium containing 3% (w/v) alginate at 30°C, pH 5.0, for 2 days. (E) Effects of pH on growth, P(3HB) production, and P(3HB) content of Cobetia sp. IU180733JP01 (5-11-6-3) in the MM medium containing 3% (w/v) alginate and 6% NaCl at 30°C for 3 days. (F) Effect of pH on growth, P(3HB) production, and P(3HB) content of Cobetia sp. IU190790JP01 (5-25-4-2) in the MM medium containing 3% (w/v) alginate and 5% NaCl at 30°C for 2 days. Closed bars, dry cell weight; gray bars, P(3HB) production; open bars, P(3HB) content. Dry cell weight was measured after freeze-drying. The P(3HB) content of freeze-dried cells was determined by the weight of the produced P(3HB). The data represent means ± S.D (n = 3).