. 2020 Jun 23;184(1):27–38. doi: 10.1104/pp.20.00425

Table 2. Known CNGC interactions with other proteins.

TEVC, Two-electrode voltage clamp; BiFC, bimolecular fluorescence complementation; CoIP, co-immunoprecipitation; nd, not determined.

Clade	Isoform	Interactor	Position	Effect	Observation	Technique	Reference
I	CNGC12	CaM	NT CaM-BD	De-activate	Deletion causes cell death	Transient expression	DeFalco et al., 2016
	CNGC11/12	CaM1	IQ domain	Activate	Mutation abolishes cell death	TEVC (X. laevis)	Zhang et al., 2019
	CNGC11/12	Apo-CaM1	IQ domain	Activate	Mutation abolishes cell death	TEVC (X. laevis)	DeFalco et al., 2016
II	CNGC6	CaM2,3,5,7	IQ domain	Negative	Plasma membrane Ca²⁺ conductance after heat shock	Whole-cell voltage patch clamping of protoplasts	Niu et al., 2020
III	CNGC14	CaM7	CT	Negative	Inhibition of Ca²⁺ influx	TEVC (X. laevis)	Zeb et al., 2020
	OsCNGC9	OsRLCK185	CT	Positive	Phosphorylation	In vitro phosphorylation assay	Wang et al., 2019
	OsCNGC9	OsRLCK185	CT	Positive	Increased [Ca²⁺]cyt	Ca²⁺ imaging in HEK cells	Wang et al., 2019
	CNGC18	CNGC7/8	CT	Negative	Inhibition of Ca²⁺ influx	TEVC (X. laevis)	Pan et al., 2019
		Apo-CaM2	IQ domain	Positive	Non-Ca²⁺ binding; CaM activates	TEVC (Xenopus laevis)	Zhou et al., 2014
		Ca²⁺ CaM2		Negative	Release of Ca²⁺ CaM2	Microscale thermophoresis	Meng et al., 2020
		CPK32		Activate	Increased Ca²⁺ influx	TEVC (X. laevis)
		MLO5/9			Increased pollen tube width	In vivo
		MLO5/9			Increased pollen tube width	Pull-down, split-ubiquitin Y2H
	MtCNGC15a, b, c	MtDMI1	NT	Neutral	Simultaneous activation	Y2H, BiFC	Charpentier et al., 2016
	CNGC15	DMI1	NT	Neutral	Simultaneous activation	BiFC	Leitão et al., 2019
	CNGC17	BAK1	nd	nd	Impaired PSK response in knockout	Split-ubiquitin Y2H	Ladwig et al., 2015
	CNGC17	AHA1, AHA2	nd	nd	Impaired PSK response in knockout	FLIM	Ladwig et al., 2015
IVa	CNGC19	BAK1/SERK4	CT	nd	Phosphorylation leads to turnover	CoIP	Yu et al., 2019
	CNGC19	CaM2, 3, 6, 7	CT	ND	Phosphorylation leads to turnover	Y2H, BiFC	Meena et al., 2019
	CNGC20	BAK1/SERK4	CT	Negative	Phosphorylation leads to turnover	Mass spectrometry	Yu et al., 2019
	CNGC20	CaM2	IQ domain	nd	Phosphorylation leads to turnover	Y2H, BiFC	Fischer et al., 2013
IVb	CNGC2	BIK1	IQ domain	?	?	CoIP, but no phosphorylation	Tian et al., 2019
	CNGC2	CaM7	IQ domain	Negative	Inhibition of Ca²⁺ influx	TEVC (X. laevis)	Tian et al., 2019
	CNGC4	BIK1	CT	Activate	Phosphorylation	Patch-clamp of protoplasts	Tian et al., 2019
		CaM7	(and NT?)	Negative	Inhibition of Ca²⁺ influx	Mass spectrometry	Chin et al., 2013; Tian et al., 2019
		CNGC2	IQ domain	Positive	Required for activity	TEVC (X. laevis)
		CNGC2	nd	Positive	Required for activity	TEVC (X. laevis)