Figure 2.

Cuticle-dependent leaf permeability in the fdl1-1 mutant. A to C, The chlorophyll leaching assay was performed on the second fully expanded leaf of 14-d-old fdl1-1 and B73 wild-type (WT) control plants (A), the second, third, and fourth fully expanded leaves of fdl1-1 and wild-type control plants (B), and the second fully expanded leaf of fdl1-1 and wild-type plants in different genetic backgrounds (C). The data in B and C are from day 3 of the chlorophyll leaching assay. Values represent the mean ± se of a minimum of five replicates. D, Temperature of the second fully expanded leaf in 14-d-old wild-type and fdl1-1 homozygous plants. Values represent the mean ± se of five biological replicates. E and F, Representative images of the leaf temperature acquired with the thermographic camera in wild-type (E) and homozygous fdl1-1 (F) plants. G, Percentage of water loss in detached 10-d-old homozygous fdl1-1 and wild-type seedlings. Values represent the mean ± se of 13 and 21 biological replicates for wild-type and fdl1-1 homozygous plants, respectively. Significant differences were assessed by Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001; ns, not significant). FW, Fresh weight.