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. 2020 Jul 13;184(1):283–299. doi: 10.1104/pp.20.00445

Figure 4.

Figure 4.

Subcellular localization and expression pattern of OsABCI7. A, GFP signals of different constructs in rice protoplasts. The full OsABCI7 protein contains 276 amino acids. ΔOsABCI7 represents the mutant OsABCI7 protein. Bars = 5 μm. B, Immunoblot analysis of OsABCI7. Protein samples were from OsABCI7 (fused HA-tag) overexpression seedlings. Antiserum against HA-tag (anti-HA), D2 (thylakoid marker), and β-actin were used in blotting. TP, Total protein; Chl, chloroplast; Thy, thylakoid membrane fraction; Str, stroma fraction; WT, wild type. C to N, GUS staining of OsABCI7Pro::GUS transgenic plants. Root (C), leaf blade (D), node V (E), culm (F), leaf sheath (G), leaf blade transverse slice (H), stamen (I), and seeds at different stages of development (J–N). Bars = 1 mm (B–F and H–M) and 100 μm (G). O, Relative expression of OsABCI7 in various organs at different growth stages. Data are means ± sd (n = 3).