Figure 5.

Phenotypic characterization of wild type (WT) and cnl1 at different temperatures and light-dependent chlorophyll synthesis of wild type and cnl1. A to C, Phenotypic comparison of wild-type and cnl1 seedlings at different temperatures. The wild type and cnl1 were grown in growth chambers after germination for 10 d at 30°C (A) and 25°C (B) and 16 d at 20°C (C) with constant temperatures and 14 h light/10 h dark cycles (400 μmol m⁻² s⁻¹). Bars = 2 cm. D, Kinetic analysis of OsABCI7 expression in wild type at 10°C. Data are means ± sd (n = 3). Asterisks indicate significant difference by Student’s t test (**P < 0.01). E to G, Ultrastructure of chloroplasts in mesophyll cells of wild type and cnl1 at different temperatures. All the cells were from the fully emerged third leaves of wild type and cnl1 seedlings at the three-leaf stage. Bars = 2 μm (in cell view) and 1 μm (in enlarged view). H to L, Chlorophyll contents (H), greening speed (I–K), and OsABCI7 expression (L) of etiolated wild-type and cnl1 seedlings during the greening process. Seven-day-old etiolated seedlings were exposed to light (250 μmol m⁻² s⁻¹) for 12 h. Bars = 2 cm. Data are means ± sd (n = 3). Different letters indicate significant differences by one-way ANOVA and Duncan’s test (P < 0.05).