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. 2020 Jul 17;184(1):236–250. doi: 10.1104/pp.19.01209

Figure 7.

Figure 7.

GmPHR1 and GmPHR4 genes regulate GmPHT1 gene expression in soybean nodules. A, GmPHR1 gene enhances GmPHT1;1 expression under low-Pi conditions (LP), while GmPHR4 gene enhances GmPHT1;1 expression independent of the availability of Pi. B, GmPHR1 enhances while GmPHR4 represses GmPHT1;11 expression in soybean nodules independent of the availability of Pi. Evidence from overexpression of GmPHR1 or GmPHR4 was consistent with data from knocking out these genes using the CRISPR/Cas9 approach. C, Both GmPHR1 and GmPHR4 genes regulate GmPHT1;4 expression in soybean nodules depending on the availability of Pi. All statistical data show means ± sd from at least three biological replicates with six individual plants. Statistical analysis was performed using Student’s t test (*P < 0.05 and **P < 0.01). Normalization of the data was performed using the expression of the empty vector control under high-Pi conditions (HP) as a reference.