Figure 5.

ZmCCD10a expression and localization. A, Relative expression of ZmCCD10a in different maize organs/tissues. The gene expression level in the root was normalized to one for comparison with that in the other organs/tissues. B, Relative abundance of ZmCCD10a transcripts under LN (low nitrogen), LP, LK (low potassium), 100 mm NaCl, and PEG (5% [w/v] PEG6000) 10 d after the treatment compared with the control (Ctrl). C, Stimulation of ZmCCD10a expression during the 25-d LP treatment. Error bars represent the se of four biological replicates. Asterisks indicate significant difference as determined by one-way ANOVA (**P < 0.01). D, ZmCCD10a expression in the primary root tip in maize by in situ hybridization. Negative control (NC) was hybridized with the sense probe. ZmCCD10a antisense probes were applied to the longitudinal and cross section of the maize root. P, Pericycle; M, meristem. Scale bars = 500 μm. E, Subcellular localization of GFP-tagged ZmCCD10a. GFP protein (plasmid University of California [pUC]-GFP) and GFP-tagged ZmCCD10a (ZmCCD10a-GFP) were transiently expressed in maize mesophyll protoplasts. GFP signals in green, auto-fluorescent signals of chloroplasts in red, and merged signals in yellow. Scale bars = 5 μm.