Figure 5.

Endogenous phytohormone contents and exogenous phytohormone sensitivity test of OsGRF7 transgenic lines. A and B, Quantification of GA (A) and IAA (B) derivatives in OsGRF7 transgenic seedlings detected with liquid chromatography-tandem mass spectrometry. Values are means ± sd of three biological replicates. Asterisks indicate significant difference by two-tailed Student’s t test (*P < 0.05). F.W., Fresh weight; ICA, indole-3-carboxaldehyde; ME-IAA, methyl indole-3-acetate; NAA, 1-naphthylacetic acid; nd, not detected (below the detection limit); WT, wild type. C, Immunohistochemical observation of IAA at the lamina joint of OsGRF7 transgenic lines. Anti-IAA antibody and Alexa 488-conjugated goat anti-rabbit IgG antibody were used. Bars = 100 μm. D, Phenotypes of OsGRF7 transgenic seedlings treated by different concentrations of phytohormones. The same control was used for 0 μm IAA and GA. Bars = 2 cm. E, Effects of different concentrations of IAA on primary root length in the wild type, GRF7OE-1, and GRF7RNAi-1. Values are means ± sd (n = 15, three biological replicates and five plants per replicate). F, Effects of different concentrations of GA₃ on the second sheath length in wild-type, GRF7OE-1, and GRF7RNAi-1 seedlings. Values are means ± sd (n = 15, three biological replicates and five plants per replicate). In E and F, different letters denote significant differences (P < 0.05) from Duncan’s multiple range test.