Figure 3.

CRY2 physically interacts with TOE1 and TOE2 in plant cells. A, Protein colocalization assays indicating the interactions of CRY2 with TOE1 and TOE2 in N. benthamiana cells. The constructs encoding TOE1 and TOE2 proteins fused to CFP and those encoding CRY2 proteins fused to YFP were cotransformed into N. benthamiana leaf epidermal cells, and TOE1 and TOE2 proteins were localized to the same NBs of CRY2 proteins. The images show overlays of fluorescence views. Scale bars = 2 μm. B to D, Split-LUC complementation imaging assays indicating the interactions of CRY2 (B), CNT2 (C), and CCT2 (D) with TOE1 in N. benthamiana cells. Empty vectors were used as negative controls. E to G, Split-LUC complementation imaging assays indicating the interactions of CRY2 (E), CNT2 (F) and CCT2 (G) with TOE2 in N. benthamiana cells. Empty vectors were used as negative controls.