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. 2020 Jul 7;184(1):316–329. doi: 10.1104/pp.20.00258

Figure 1.

Figure 1.

Characterization of carp9 mutants. A, Gene structure of CARP9 showing single nucleotide deletion of the carp9-1 allele and T-DNA insertion sites in carp9-2, carp9-3, carp9-4, and carp9-5. Black boxes and lines represent exons and introns, respectively; gray boxes represent coding sequence, while blue boxed shows 5′ and 3′ UTR regions. A white asterisk marks the position of the stop codon that results from the frameshift caused by the carp9-1 single nucleotide deletion (G2464Del). B, Phenotypic characterization of carp9 mutants, control lines (reporter and Col-0), and carp9-1 mutants complemented by the overexpression of the CARP9 cDNA (carp9-1; Pro35S:CARP9). Twenty-one-day-old plants, fully expanded siliques, and 40-d-old plants are displayed. Bars = 1 cm. Plants were imaged individually, digitally extracted, and mounted on a single black background panel to facilitate comparison and observation. C, Analysis of the flowering time of control, carp9 mutant, and complemented lines grown in long-day photoperiod as measured by the number of rosette leaves or the number of days to bolting. Error bars show means ± se (n ≥ 15). D, Bioluminescence activity, quantified by a luminometer, of 12 leave discs belonging to 20-d-old carp9-1 mutants and reporter plants. Error bars show means ± se (n ≥ 12). Asterisks indicate significance by two-tailed, unpaired t test Holm-Sidak corrected (*P < 0.05). E, Bioluminescence activity as measured with a CCD camera, in 20-d-old carp9-1 mutants and reporter plants. Luminescence intensity is color scaled from low (blue) to high (white). Two pots containing 12 plants each were imaged individually, digitally extracted, mounted in a single black background panel, and displayed in the figure. F, RNA blots for detecting amiRLuc. U6 was used as a loading control. G, Phenotypic features of 18-d-old carp9 mutants, control lines, and carp9-1/carp9-2 compound heterozygous mutants. Bar = 1 cm. Plants were imaged individually, digitally extracted, and mounted in a single black background panel to facilitate comparison and observation.