Table 2. Identity of RNAi sequences cloned into the TRV2 vector against the N. benthamina PDS gene and their off-target genes predicted by pssRNAit at default parameters.

The National Center for Biotechnology Information accession number for the full-length NbPDS sequence used in this study is DQ469932.1.

Name of Construct	Range of Target Sequence	Length	No. of siRNAs	Construct Sequences	No. of Off-Targets	Significant Off-Targets (No. of Hits)
Ps1058	1058–1250	193	13	TGA​ATG​AGG​ATG​GAA​GTG​TCA​AAT​GTT​TTA​TAC​TGA​ATA​ATG​GCA​GTA​CAA​TTA​AAG​GAG​ATG​CTT​TTG​TGT​TTG​CCA​CTC​CAG​TGG​ATA​TCT​TGA​AGC​TTC​TTT​TGC​CTG​AAG​ACT​GGA​AAG​AGA​TCC​CAT​ATT​TCC​AAA​AGT​TGG​AGA​AGC​TAG​TGG​GAG​TTC​CTG​TGA​TAA​ATG​TCC​ATA​TAT​GGT​TTG​A	239	Niben101Scf04220g03010.1 (2)
						Niben101Scf01732g02009.1 (2)
Ps592	592–782	191	13	ATG​CCT​AAC​AAG​CCA​GGG​GAG​TTC​AGC​CGC​TTT​GAT​TTT​CCT​GAA​GCT​CTT​CCT​GCG​CCA​TTA​AAT​GGA​ATT​TTG​GCC​ATA​CTA​AAG​AAC​AAC​GAA​ATG​CTT​ACG​TGG​CCC​GAG​AAA​GTC​AAA​TTT​GCT​ATT​GGA​CTC​TTG​CCA​GCA​ATG​CTT​GGA​GGG​CAA​TCT​TAT​GTT​GAA​GCT​CAA​GAC​GGT​TTA​AG	199	Niben101Scf09867g02016.1 (2)
						Niben101Scf06725g02017.1 (2)
Ps415	415–612	198	13	CTG​GAG​GCA​AGA​GAT​GTC​CTA​GGT​GGG​AAG​GTA​GCT​GCA​TGG​AAA​GAT​GAT​GAT​GGA​GAT​TGG​TAC​GAG​ACT​GGG​TTG​CAC​ATA​TTC​TTT​GGG​GCT​TAC​CCA​AAT​ATG​CAG​AAC​CTG​TTT​GGA​GAA​CTA​GGG​ATT​GAT​GAT​CGG​TTG​CAG​TGG​AAG​GAA​CAT​TCA​ATG​ATA​TTT​GCG​ATG​CCT​AAC​AAG​CCA​GGG​GAG	203	Niben101Scf18745g01012.1 (2)
						Niben101Scf10385g00005.1 (2)
						Niben101Scf17044g01001.1 (2)
						Niben101Scf03965g00005.1 (2)
						Niben101Scf00397g08002.1 (2)
Ps372	372–568	197	14	TAC​AGC​AAA​ATA​TCT​GGC​AGA​TGC​TGG​TCA​CAA​ACC​GAT​ATT​GCT​GGA​GGC​AAG​AGA​TGT​CCT​AGG​TGG​GAA​GGT​AGC​TGC​ATG​GAA​AGA​TGA​TGA​TGG​AGA​TTG​GTA​CGA​GAC​TGG​GTT​GCA​CAT​ATT​CTT​TGG​GGC​TTA​CCC​AAA​TAT​GCA​GAA​CCT​GTT​TGG​AGA​ACT​AGG​GAT​TGA​TGA​TCG​GTT​GCA​GTG​GA	233	Niben101Scf10385g00005.1 (2)
						Niben101Scf17044g01001.1 (2)
						Niben101Scf03965g00005.1 (2)
NbPDS PssRNAit	1279–1477	119	17	CTG​CTC​TTC​AGC​AGA​AGC​CCG​TTG​CTC​AGT​GTG​TAC​GCT​GAC​ATG​TCT​GTT​ACA​TGT​AAG​GAA​TAT​TAC​AAC​CCC​AAT​CAG​TCT​ATG​TTG​GAA​TTG​GTA​TTT​GCA​CCC​GCA​GAA​GAG​TGG​ATA​AAT​CGT​AGT​GAC​TCA​GAA​ATT​ATT​GAT​GCT​ACA​ATG​AAG​GAA​CTA​GCG​AAG​CTT​TTC​CCT​GAT​GAA​ATT​TCG​GCA​G	265	Niben101Scf05449g01006.1 (2)
						Niben101Scf00185g04002.1 (2)
						Niben101Scf04992g03010.1 (2)
						Niben101Scf00167g00004.1 (2)
						Niben101Scf03283g04019.1 (2)
						Niben101Scf13640g00006.1 (2)