Figure 2.

A proposed mechanism of unidirectional scaffold switching for Plk4 from the Cep192-bound state to the Cep152-bound state. In early G1, Cep192 localized around a daughter centriole recruits Plk4 at its outer end. As Cep152 (presumably as a dimeric Cep63-Cep152 complex or trimeric Cep57-Cep63-Cep152) is recruited, Cep152 snatches Plk4 away from the Cep192 scaffold (the inset diagrams) and assembles around the Cep192 layer in late G1, prompting the repositioning of Plk4 to the outer edge of the Cep152 scaffold. Note that because Cep57, Cep152 and inactivated Plk4 show a localization pattern of a ninefold radial symmetry [48,49], the Cep63-Cep152 assemblies are depicted in clusters.