Table 3.
mRNA Expression of Human SLC51A and SLC51B in Various Cell Lines.
| SLC51A Level | SLC51B Level | |||||
|---|---|---|---|---|---|---|
| Cell Line | TPM | pTPM | NX | TPM | pTPM | NX |
| A-431 | 0.4 | 0.5 | 1.0 | 0.0 | 0.0 | 0.0 |
| A549 | 0.7 | 0.8 | 1.6 | 1.3 | 1.6 | 1.4 |
| AF22 | 0.6 | 0.8 | 1.4 | 0.2 | 0.3 | 0.2 |
| AN3-CA | 0.7 | 0.9 | 1.7 | 0.7 | 0.9 | 0.7 |
| ASC diff | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| ASC TERT1 | 0.3 | 0.3 | 1.0 | 0.1 | 0.1 | 0.1 |
| BEWO | 0.0 | 0.0 | 0.1 | 0.4 | 0.5 | 0.4 |
| BJ | 0.3 | 0.4 | 0.8 | 0.0 | 0.0 | 0.0 |
| BJ hTERT+ | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| BJ hTERT+ SV40 Large T+ | 0.0 | 0.0 | 0.1 | 0.2 | 0.2 | 0.2 |
| BJ hTERT+ SV40 Large T+ RasG12V | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| Caco-2 | 0.2 | 0.3 | 0.7 | 14.9 | 18.3 | 16.5 |
| CAPAN-2 | 0.2 | 0.2 | 0.4 | 0.0 | 0.1 | 0.0 |
| Daudi | 0.0 | 0.0 | 0.1 | 0.0 | 0.1 | 0.1 |
| EFO-21 | 0.8 | 1.0 | 1.9 | 11.0 | 13.5 | 11.1 |
| fHDF/TERT166 | 0.4 | 0.4 | 1.1 | 0.0 | 0.0 | 0.0 |
| HaCaT | 0.3 | 0.4 | 0.9 | 0.0 | 0.0 | 0.0 |
| HAP1 | 0.0 | 0.1 | 0.2 | 0.0 | 0.0 | 0.0 |
| HBEC3-KT | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| HBF TERT88 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| HDLM-2 | 0.3 | 0.4 | 0.7 | 0.1 | 0.1 | 0.1 |
| HEK 293 | 1.2 | 1.5 | 2.8 | 0.2 | 0.3 | 0.2 |
| HEL | 0.4 | 0.5 | 1.0 | 0.0 | 0.0 | 0.0 |
| HeLa | 0.2 | 0.3 | 0.8 | 0.1 | 0.1 | 0.1 |
| HepaRG | Detected* | Detected* | ||||
| HepG2 | 0.5 | 0.7 | 1.7 | 10.4 | 12.7 | 13.8 |
| HHSteC | 0.1 | 0.1 | 0.2 | 0.1 | 0.1 | 0.1 |
| HL-60 | 0.5 | 0.6 | 1.4 | 0.1 | 0.2 | 0.2 |
| HMC-1 | 0.5 | 0.7 | 1.4 | 0.0 | 0.0 | 0.0 |
| HSkMC | 0.1 | 0.1 | 0.3 | 0.0 | 0.0 | 0.0 |
| hTCEpi | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| hTEC/SVTERT24-B | 0.0 | 0.1 | 0.2 | 0.1 | 0.1 | 0.1 |
| hTERT-HME1 | 0.4 | 0.5 | 1.3 | 0.0 | 0.0 | 0.0 |
| HuH-7 | Detected* | Detected* | ||||
| HUVEC TERT2 | 0.0 | 0.1 | 0.2 | 0.0 | 0.0 | 0.0 |
| K-562 | 0.1 | 0.1 | 0.2 | 0.2 | 0.2 | 0.2 |
| Karpas-707 | 1.0 | 1.7 | 3.3 | 0.1 | 0.2 | 0.2 |
| LHCN-M2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| MCF7 | 0.2 | 0.3 | 0.6 | 0.3 | 0.4 | 0.4 |
| MOLT-4 | 0.0 | 0.0 | 0.1 | 0.0 | 0.1 | 0.0 |
| NB-4 | 0.3 | 0.4 | 1.2 | 0.2 | 0.3 | 0.3 |
| NTERA-2 | 0.0 | 0.0 | 0.1 | 0.4 | 0.5 | 0.4 |
| PC-3 | 0.8 | 1.0 | 2.0 | 0.4 | 0.5 | 0.4 |
| REH | 3.4 | 4.4 | 7.9 | 0.0 | 0.0 | 0.0 |
| RH-30 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 0.0 |
| RPMI-8226 | 0.1 | 0.2 | 0.5 | 0.6 | 0.8 | 0.8 |
| RPTEC TERT1 | 0.0 | 0.0 | 0.0 | 0.3 | 0.3 | 0.4 |
| RT4 | 0.3 | 0.4 | 0.9 | 0.0 | 0.0 | 0.0 |
| SCLC-21H | 0.2 | 0.2 | 0.3 | 0.8 | 1.0 | 0.6 |
| SH-SY5Y | 0.2 | 0.3 | 0.5 | 0.5 | 0.7 | 0.5 |
| SiHa | 0.2 | 0.3 | 0.5 | 0.0 | 0.0 | 0.0 |
| SK-BR-3 | 1.1 | 1.3 | 3.2 | 0.0 | 0.0 | 0.0 |
| SK-MEL-30 | 0.1 | 0.1 | 0.3 | 0.0 | 0.0 | 0.0 |
| T-47d | 1.0 | 1.2 | 2.3 | 0.4 | 0.5 | 0.4 |
| THP-1 | 0.1 | 0.2 | 0.5 | 0.0 | 0.0 | 0.0 |
| TIME | 0.2 | 0.2 | 0.5 | 0.0 | 0.0 | 0.0 |
| U-138 MG | 0.7 | 0.9 | 1.6 | 0.3 | 0.3 | 0.3 |
| U-2 OS | 0.3 | 0.4 | 0.7 | 0.0 | 0.0 | 0.0 |
| U-2197 | 0.5 | 0.7 | 1.3 | 0.1 | 0.1 | 0.1 |
| U-251 MG | 0.3 | 0.4 | 0.9 | 0.1 | 0.1 | 0.1 |
| U-266/70 | 1.1 | 1.8 | 3.3 | 0.1 | 0.2 | 0.2 |
| U-266/84 | 1.3 | 2.0 | 4.0 | 0.2 | 0.4 | 0.3 |
| U-698 | 0.2 | 0.2 | 0.6 | 0.1 | 0.2 | 0.2 |
| U-87 MG | 0.5 | 0.6 | 1.1 | 0.0 | 0.1 | 0.0 |
| U-937 | 0.2 | 0.3 | 0.6 | 0.1 | 0.1 | 0.1 |
| WM-115 | 0.2 | 0.3 | 0.6 | 0.1 | 0.2 | 0.1 |
Data on transcript expression levels per gene in 64 cell lines were obtained from The Human Protein Atlas version 19 and Ensembl version 92.38, and were determined using next-generation sequencing-based RNA-sequencing. Values are presented as transcripts per million (TPM) RNA molecules per sample, protein-coding transcripts per million (pTPM) and normalized expression (NX). NX data for every gene in each sample were obtained by normalizing TPM data using trimmed mean of M values (Robinson & Oshlack, 2010), followed by Pareto scaling (van den Berg, Hoefsloot, Westerhuis, Smilde, & van der Werf, 2006). A color scale was applied to each column separately, with green and red denoting the highest and lowest levels of expression, respectively.
HepaRG and HuH-7 cell lines both express SLC51A and SLC51B mRNA (Landrier, et al., 2006; Sissung, et al., 2019), but were not included in the current dataset from the Human Protein Atlas.