FIGURE 1.

Replication of DTMUV in avian and mammalian cells. (A) Replication efficiencies of DTMUV in avian and mammalian cells as determined by the copy numbers of the viral genomic RNA by RT-qPCR. BHK-21, Vero, 293T, DF-1, and DEF cells were infected with DTMUV at an MOI of approximately 1, harvested at the indicated time points, and subjected to RNA extraction. Equal amounts of total RNA were reverse-transcribed. The levels of DTMUV genomic RNA were determined by RT-qPCR, and the average value was obtained from three repeats. (B) Replication efficiencies of DTMUV in avian and mammalian cells as determined by TCID50. BHK-21, Vero, 293T, DF-1, and DEF cells were infected with DTMUV at an MOI of approximately 1, harvested at the indicated time points, and the viral titers were determined as TCID50.