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. 2020 Aug 26;11:2069. doi: 10.3389/fmicb.2020.02069

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Copyright © 2020 Xiang, Huang, Xiong, Rong, Li, Liu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Regulation of DTMUV infection-induced up-regulation of VIPERIN, IFIT5, and CMPK2 by knockdown of IRF1. The relative mRNA levels of IRF1 (A), VIPERIN (B), IFIT5 (C), and CMPK2 (D) in IRF1-knockdown 293T cells infected with DTMUV. 293T cells were transfected with siIRF1 and siNC for 6 h, respectively, and infected with DTMUV at an MOI of approximately 1. Cells were harvested at the indicated time points for RT-qPCR with gene specific primers for each of the four genes to determine the relative mRNA levels. ^∗p < 0. 05; ^∗∗p < 0. 01; ns, non-significance.