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. 2020 Jun 29;219(9):e202003091. doi: 10.1083/jcb.202003091

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© 2020 Efimova et al.

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Figure 7. — APC knockdown abrogates growth cone recovery after Sema3A-induced collapse. (A–C) Staining with phalloidin (cyan) and MAP2 antibody (red) of neurons transfected with control (A and C, left) or APC sh1 (B and C, right) shRNAs at DIV1, treated with Sema3A for 1 h at DIV7, and washed out of Sema3A for indicated periods of time. GC, growth cones. Scale bars, 5 µm (A and B) and 50 µm (C, applies to all panels). (D) Mean fluorescence intensity of phalloidin staining in indicated conditions. Median ± SEM; ***, P < 0.001; **, P < 0.01; ns, not significant; n = 51–136 growth cones from one representative experiment out of two independent experiments; Kruskal–Wallis multiple comparisons test with post hoc Dunn’s test; one additional experiment was evaluated qualitatively. Purple symbols indicate significance between shControl and shAPC for respective conditions.