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. 2020 Jul 24;219(9):e202003084. doi: 10.1083/jcb.202003084

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© 2020 Fingerhut and Yamashita

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Figure S5. — Transcripts for other axonemal, Y-linked, and spermatid proteins do not localize to kl-granules. (A–G) smFISH against other axonemal (A–C), Y-linked (D–G), or spermatid-essential (H) transcripts in wild type. (A) Dic61B (dynein intermediate chain, red) and DAPI (white). (B) CG3339 (axonemal dynein heavy chain, red) and DAPI (white). (C) CG17083 (ODA docking complex, red) and DAPI (white). (D) CCY (red) and DAPI (white). (E) PPR-Y (red) and DAPI (white). (F and G) kl-5 (red), ORY (blue), DAPI (white), and kl-granules (yellow arrows). (G) fzo (red) and DAPI (white). For all, spermatid cyst, cyan; SC nuclei, yellow dashed line; neighboring SC nuclei, white dashed line. Note that because Fzo is translated early in spermiogenesis, an SC image was used. Scale bars: 25 µm (A–F) and 10 µm (G and H).