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. 2020 Jul 22;219(9):e202003026. doi: 10.1083/jcb.202003026

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© 2020 Wang et al.

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Figure 7. — Overexpression of atoh7, otx2, and ptf1α in vsx1⁺ and OC1⁺ RPCs. (A) Representative images of vsx1⁺ cells (labeled by tdTomato-NLS) after overexpressing atoh7, otx2, and ptf1α in vsx1⁺ RPCs with no TF overexpression as the blank. Tg(ptf1α:GFP) was used to indicate cell body location. (B) Cell type distribution of vsx1⁺ cells in A. The significant increase of each neuron type compared with blank is shown. (C and D) Vsx1⁺ AC subtypes after ptf1α overexpression. Representative image of glyT1⁺ ACs is shown in C. Zoom-in images in the yellow rectangle are shown on the right side. Statistic distribution of vsx1⁺ AC subtypes is shown in D. (E) Representative images of OC1⁺ cells (labeled by tdTomato-NLS) after overexpressing atoh7, otx2, and ptf1α in OC1⁺ RPCs with no TF overexpression in the blank. (F) Cell type distribution of OC1⁺ cells in E. The significant increase of each neuron type compared with blank is shown. (G and H) OC1⁺ AC subtypes after ptf1α overexpression. The image of a gad1b⁺ AC is shown in G. Zoom-in images in the yellow rectangle are shown on the right side. Statistical distribution of OC1⁺ AC subtypes is shown in H. (I) A summary graph. Scale bar, 10 µm. **, P < 0.001; ***, P < 0.0001 (Fisher's exact test).