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. 2020 Jul 22;219(9):e202003026. doi: 10.1083/jcb.202003026

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© 2020 Wang et al.

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Figure S4. — Single-cell RNA sequencing of atoh7⁺ RPCs. (A) Time-lapse result of Tg(atoh7:turboGFP-dest1::atoh7:gapRFP) retina. TurboGFP-dest1 signal appears and disappears earlier than that of gapRFP. (B) FACS analysis of retinal cells from the Tg(atoh7:turboGFP-dest1::atoh7:gapRFP) embryos. The GFP⁺RFP⁻ cells for scRNA-seq are highlighted. (C) The t-SNE plot showing the clustering result of GFP⁺RFP⁻ cells in B and the annotation. (D) Featured genes of each cell cluster in C. (E) Cell-cycle distribution of cells in C. Cells during the same cell cycle phase tend to cluster together. (F) Four clusters of atoh7⁺ G2/M RPCs. (G) Expression patterns of cluster 2–specific (onecut1, pou2f2a, myca) and cluster 3–specific (vsx1, olig2, pou3f1) TFs of cells in F.