Table 2. Primers used to synthesize ptf1α sgRNA.

Primer	Sequence (5′ to 3′)
Forward primer 1	TGT​AAT​ACG​ACT​CAC​TAT​AGG​TAG​TCG​GAT​ATC​ACA​GGA​GTT​TTA​GAG​CTA​GAA​AT
Forward primer 2	TGT​AAT​ACG​ACT​CAC​TAT​AGG​GGC​TTA​CTG​AAG​AGG​CGG​GTT​TTA​GAG​CTA​GAA​AT
Forward primer 3	TGT​AAT​ACG​ACT​CAC​TAT​AGG​CAG​TCG​GAC​ATG​CCA​ATT​GTT​TTA​GAG​CTA​GAA​AT
Forward primer 4	TGT​AAT​ACG​ACT​CAC​TAT​AGG​AAG​TCG​TCT​GTG​TCC​AAG​GTT​TTA​GAG​CTA​GAA​AT
Reverse primer	AAA​AAA​AGC​ACC​GAC​TCG​GTG​CCA​C