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Example trajectory of Swi6-mEos3.2 in a live yeast cell nucleus. Frametime is 30 ms. Video was made using the MTT software (Sergé et al., 2008) on one of the live cell PALM FOV acquisitions. Pixel size was 120 nm. Frames are colored from blue to red as time increases. Both free and confined diffusion are apparent in this trajectory.
