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. 2020 Jul 17;219(9):e202001120. doi: 10.1083/jcb.202001120

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© 2020 Chiusa et al.

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Figure 2. — Increased nuclear FUS in Dsk5 heterozygote mice expressing constitutively active EGFR kinase. (A and B) pEGFR and total EGFR in kidney tissue lysates (20 µg/lane) from four WT and four Dsk5 heterozygote (Dsk5^+/−) mice were analyzed by Western blotting. pEGFR and EGFR bands were quantified by densitometry analysis, and values are expressed as pEGFR/EGFR ratio. Circles represent single mice (four WT and four Dsk5^+/−) and bars represent mean ± SD. (C) Immunohistochemical detection of FUS in paraffin kidney sections from control (WT) and Dsk5^+/− male mice. Increased nuclear FUS is evident in Dsk5^+/− mice. (D) Glomerular FUS-positive cells as well as total cells were counted, and values are expressed as percentage of FUS-positive cells/glomerulus. Circles represent single glomerular values (57 WT and 82 Dsk5^+/−) and bars represent mean ± SD (n = 3 mice). (E) Nonnuclear and nuclear fractions (20 µg/lane) of kidneys from four WT and four Dsk5^+/− mice were analyzed by Western blot for levels of FUS, GAPHD (nonnuclear marker), and Histone 3 (H3, nuclear marker). (F) FUS and H3 bands were quantified by densitometry, and values are expressed as FUS/H3 ratio. Circles represent single mice (four WT and four Dsk5^+/−) and bars represent mean ± SD. Two-tailed t test was used for statistical analysis.