Video 1.
TKS5 and FGD1 colocalize in dynamic collagen-remodeling invadopodia. Left panel: MDA-MB-231 cells expressing FGD1GFP (green) and TKS5mCherry (red) were plated on top of a thin type I collagen layer (gray). A time-lapse sequence was started after 45 min by confocal spinning-disk microscopy. Images were taken every min during 45 min. The right panel shows the active remodeling of the collagen fibers by the TKS5 FGD1 double-positive cell that can be compared with collagen remodeling by two nontransfected cells visible on the upper and lower right corners. Frame rate of the video is 6 frames/s.