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. 2020 Jun 4;219(9):e201905199. doi: 10.1083/jcb.201905199

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© 2020 Sánchez-Huertas et al.

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Figure S2. — Overexpression of NAV1 compacts the GC and inhibits its dynamics. Related to Fig. 1. Axonal GCs of cortical neurons (2 DIV) transfected with Lifeact-Tq and GFP or GFP-NAV1, and imaged by time-lapse microscopy during 2 min. (A) Lifeact-Tq images of GCs of control and NAV1-overexpressing neurons. Scale bar, 2 µm. (B) Quantification of GC area. (C) Color-coded time-lapse projections of the Lifeact-Tq signal. The indicated time points were used. Arrowheads mark filopodia. Scale bar, 2 µm; time, seconds. (D) GC dynamics in control and NAV1-overexpressing neurons. n = 30 (GFP) and 33 (GFP-NAV1) GCs from three independent experiments. Dot plot shows means. Histogram shows means ± SEM. Analyzed by two-tailed Student's t test.