Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 4;219(9):e201905199. doi: 10.1083/jcb.201905199

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Sánchez-Huertas et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S3. — NAV1 depletion impairs netrin-1–evoked axon branching. Related to Fig. 2. Cortical neurons transfected with the indicated shRNAs at 1 DIV, treated with netrin-1 (500 ng/ml) for 24 h, and fixed at 3 DIV. (A) Anti-mCherry immunostaining of neurons transfected with shRNA-luc and either left untreated or treated with netrin-1. Scale bar, 20 µm. (B) Quantitative analysis of branch points per axon. n = 60 (shRNA-luc), 59 (shRNA-luc + netrin-1), 55 (shRNA-NAV1), and 49 (shRNA-NAV1 + netrin-1) neurons from two independent experiments. Histogram shows means ± SEM. Analyzed by two-tailed Student's t test.