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GFP-NAV1 tracking a polymerizing MT plus end inside a filopodium turns into a pausing GFP-NAV1 granule devoid of EB3-mCherry. Related to Fig. 5 C. Time-lapse microscopy of a GC filopodium from a neuron transfected with GFP-NAV1, EB3-mCherry, and Lifeact-Tq. Overlays of Lifeact/EB3 (top) and NAV1/EB3 (bottom) are shown. From the 45th second of the movie on, the EB3 signal disappears while the NAV1 granule signal persists. Time interval: 1 second.
