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GFP-NAV1 persists at the tip of nonpolymerizing MTs within the F-actin–rich domain of GCs. Related to Fig. 5 E. Time-lapse microscopy of the axonal GC of a neuron transfected with GFP-NAV1 and Lifeact-Tq, with MTs labeled by SiR-tubulin (red). Overlays of NAV1/MTs (left) and NAV1/MTs/F-actin (right) are shown. The F-actin network is pulled backward by the translocating NAV1-labeled MTs. Time interval: 1 second.
