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. 2020 Jun 4;219(9):e201905199. doi: 10.1083/jcb.201905199

Table 2. Oligonucleotides used in this study.

Construct Oligonucleotide (5′–3′)
GFP-NAV1-SRIP/SRNN CCT​GGG​AAA​GCC​GAG​CCG​GAA​CAA​TCG​AGG​ACC​CTA​TGC​GGA​GGT​C
GAC​CTC​CGC​ATA​GGG​TCC​TCG​ATT​GTT​CCG​GCT​CGG​CTT​TCC​CAG​G
GFP-NAV1-SGIP/SGNN GAT​CCA​GAA​GTC​CTC​AGG​CAA​CAA​TGT​CAA​GCC​AGT​AAA​TGG​GCG
CGC​CCA​TTT​ACT​GGC​TTG​ACA​TTG​TTG​CCT​GAG​GAC​TTC​TGG​ATC
GFP-NAV1-N1 GCT​GTA​CAA​GTC​CGG​ACT​GCC​CAA​GCG​CGC​CAA​GG
GAG​ATC​TGA​GTC​CGG​ATT​ACT​TGT​CAA​GAT​TGG​CTA​GTG​AGG
GFP-NAV1-N3 TCT​TAG​ATC​TCA​TAT​GGA​GTC​CCA​GAG​AAA​GAG​G
TCT​TGT​CGA​CCT​ACT​TGT​CAA​GAT​TGG​CTA​G
GFP-NAV1-N4 GGT​TCC​GGA​CCA​GAG​TCC​CAG​AGA​AAG
AGG​TGT​CGA​CTT​AAG​GTT​TGC​CTG​CGA​CTT​T
GFP-NAV1-N5 GCT​GTA​CAA​GTC​CGG​ACT​GGC​AGA​AAG​TGG​GCT​GAG
GAG​ATC​TGA​GTC​CGG​ATT​ACT​TGT​CAA​GAT​TGG​CTA​GTG​AGG
GFP-NAV1-C1 GCT​GTA​CAA​GTC​CGG​AGT​CAA​CTC​CAA​CAG​TCT​GGA​TC
GAG​ATC​TGA​GTC​CGG​ATT​AAA​GTG​TTG​CCT​GAA​GGT​TGG​G
His-NAV1-N1 CAG​CCA​TAT​GGC​TAG​CCT​GCC​CAA​GCG​CGC​CAA​GG
CAC​CAG​TCA​TGC​TAG​CTT​ACT​TGT​CAA​GAT​TGG​CTA​GTG​AGG
His-NAV1-N5 CAG​CCA​TAT​GGC​TAG​CCT​GGC​AGA​AAG​TGG​GCT​GAG
CAC​CAG​TCA​TGC​TAG​CTT​ACT​TGT​CAA​GAT​TGG​CTA​GTG​AGG
His-NAV1-N6 TCT​TAG​ATC​TCA​TAT​GCG​CAA​GAC​TAG​CTT​AGA​TGT
TCT​TGT​CGA​CCT​ACT​TGT​CAA​GAT​TGG​CTA​G
shRNA-NAV1#1 (pLL3.7) TGC​CTA​GAA​CAG​CTA​CTC​TTC​TTC​AAG​AGA​GAA​GAG​TAG​CTG​TTC​TAG​GCT​TTT​TTC
TCG​AGA​AAA​AAG​CCT​AGA​ACA​GCT​ACT​CTT​CTC​TCT​TGA​AGA​AGA​GTA​GCT​GTT​CTA​GGC​A
shRNA-NAV1#2 (pLL3.7) TGC​CAT​GAT​GCA​GCA​CTA​CAT​TTC​AAG​AGA​ATG​TAG​TGC​TGC​ATC​ATG​GCT​TTT​TTC
TCG​AGA​AAA​AAG​CCA​TGA​TGC​AGC​ACT​ACA​TTC​TCT​TGA​AAT​GTA​GTG​CTG​CAT​CAT​GGC​A
shRNAs (pAAV-CAG-YFP) TTG​GTA​CCC​AGC​AGA​GAT​CCA​GTT​TGG
TTG​GTA​CCG​AAG​CGA​GCT​TAT​CGA​TAC​C