Video 1.
Intravital imaging of 690.cl2 cells expressing Cdc42ep5 in a living tumor. 690.cl2KO cells ectopically expressing GFP-Cdc42ep5 (green) were grown subcutaneously and imaged in vivo using two-photon microscopy, which enabled the acquisition of a second harmonic signal (collagen fibers, magenta). Images were acquired every 14 min for a total of 2 h 45 min. Scale bar, 100 µm. Middle panels show magnifications of indicated areas showing fast amoeboid movement into the matrix. Right panels represent motion analysis images of the same area generated by overlaying blue, green, and red images from different time points. Distinct areas of color indicate motile cells, whereas static regions appear white.