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. 2020 Jun 23;219(9):e202004191. doi: 10.1083/jcb.202004191

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© 2020 Grond et al.

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Figure 1. — Genomic GORASP2 locus in the cDKO small intestine (si) in control and TAM-treated tissue. (A) Schematic of the GORASP2 KI allele with the LacZ/Neo cassette between exons 4 and 5. (B) Schematic of the GORASP2 cFlox allele upon crossing GORASPKI with Flp-recombinase expressing mice. The exon 5 is now flanked by two LoxP sites. (C) Schematic of the GORASP2 FLOX allele upon TAM injection (after crossing with a Rosa Cre ERT2 mouse). Exon 5 is excised. (D) A series of PCRs using different combinations of primers showing that exon 5 is deleted (a–d), a part of intron 5 is still present (e and f), and exons 6 and 7 (g and h) and exons 8 and 9 (i and j) are still present in genomic DNA.