Table 1. Primers for PCR shown in Figs. 1 and S1.
Name | Sequence (5′ to 3′) | Annealing | |
---|---|---|---|
a | F-GORASP2 ex4 | CCGATCCTTTCCTCAGCCGTCAGC | 62.0°C |
b | R-GORASP2 ex4LoxP | TGTGGAGTCCTTCCTTGCTC | |
c | F-GORASP2 ex3 | GGGTTAGCATTCGTTTCTGC | 53.4°C |
d | R-GORASP2 ex5 | CATGCAGAGTTTGGTGTGATG | |
e | F-GORASP2 int5 | GAGCAAGGAAGGACTCCACA | 56.8°C |
f | R-GORASP2 int5 | CAAGAGGCCGAGATCCTGAG | |
g | F-GORASP2 ex6 | CGAATACCTACACGCCCCTT | 57.2°C |
h | R-GORASP2 ex7 | TGCCGAGCTAATGGAGAGTC | |
i | F-GORASP2 ex8 | ACTGTACCATTGCCGCC | 58.4°C |
j | R-GORASP2 ex9 | GAGCTCTGGCAAACCGACC | |
k | F-GORASP2 ex4 | AATCCAATTCCCCAGCG | 57.7°C |
l | R-GORASP2 ex6 | GGGGGTACCAGTCATTTGTCC | |
m | F-GORASP2 ex1 | CAAGTGCCACGTCCCAAGAG | 58.5°C |
n | R-GORASP2 ex4 | CTGTGAGGTCTCAATCCTGCC | |
o | F-GORASP2 ex6 | CGAATACCTACACGCCCCTT | 57.2°C |
p | R-GORASP2 ex10 | ATGCCAGGTAAGTTTCGGGG | |
F-GAPDH ex3–4 | TGTCGTGGAGTCTACTGGTG | 53.1°C | |
R-GAPDH ex3–4 | ACACCCATCACAAACATGG |
List of primers used in this study. F, Forward; R, Reverse; int, intron; ex, exon.