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. 2020 Jun 23;219(9):e202004191. doi: 10.1083/jcb.202004191

Table 1. Primers for PCR shown in Figs. 1 and S1.

Name Sequence (5′ to 3′) Annealing
a F-GORASP2 ex4 CCG​ATC​CTT​TCC​TCA​GCC​GTC​AGC 62.0°C
b R-GORASP2 ex4LoxP TGT​GGA​GTC​CTT​CCT​TGC​TC
c F-GORASP2 ex3 GGG​TTA​GCA​TTC​GTT​TCT​GC 53.4°C
d R-GORASP2 ex5 CAT​GCA​GAG​TTT​GGT​GTG​ATG
e F-GORASP2 int5 GAG​CAA​GGA​AGG​ACT​CCA​CA 56.8°C
f R-GORASP2 int5 CAA​GAG​GCC​GAG​ATC​CTG​AG
g F-GORASP2 ex6 CGA​ATA​CCT​ACA​CGC​CCC​TT 57.2°C
h R-GORASP2 ex7 TGC​CGA​GCT​AAT​GGA​GAG​TC
i F-GORASP2 ex8 ACTGTACCATTGCCGCC 58.4°C
j R-GORASP2 ex9 GAG​CTC​TGG​CAA​ACC​GAC​C
k F-GORASP2 ex4 AATCCAATTCCCCAGCG 57.7°C
l R-GORASP2 ex6 GGG​GGT​ACC​AGT​CAT​TTG​TCC
m F-GORASP2 ex1 CAA​GTG​CCA​CGT​CCC​AAG​AG 58.5°C
n R-GORASP2 ex4 CTG​TGA​GGT​CTC​AAT​CCT​GCC
o F-GORASP2 ex6 CGA​ATA​CCT​ACA​CGC​CCC​TT 57.2°C
p R-GORASP2 ex10 ATG​CCA​GGT​AAG​TTT​CGG​GG
F-GAPDH ex3–4 TGT​CGT​GGA​GTC​TAC​TGG​TG 53.1°C
R-GAPDH ex3–4 ACA​CCC​ATC​ACA​AAC​ATG​G

List of primers used in this study. F, Forward; R, Reverse; int, intron; ex, exon.