Figure 7.

CdaA excludes the CdaI streaks from the posterior cortical domain. CdaI-GFP-expressing cells (detected with anti-GFP antibodies, red) were stained with anti-centrin antibodies (green) and DAPI (blue) after growth at either 29°C or 39°C. At 29°C, the pattern of CdaI-GFP is normal in both wild-type (A–D′) and cdaI-1 backgrounds (E–F′), except for a slight anterior displacement of the oral primordium and the division plane in wild-type background cells expressing CdaI-GFP, due to a partial loss of function of CdaI caused by addition of GFP (Jiang et al., 2017, 2019a). At 39°C in the cdaA-1 background, the CdaI-GFP streaks leak into the posterior half of the cell (G–H′). cs, cortical subdivision; ma, macronucleus; mi, micronucleus; noa, new oral apparatus (oral primordium); oa, oral apparatus. The arrowheads show the posterior ends of some CdaI-GFP streaks.