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. 2020 Jul 6;219(9):e202002077. doi: 10.1083/jcb.202002077

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© 2020 Jiang et al.

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Figure 9. — Overexpression of GFP-CdaA partially phenocopies a loss of CdaI. (A–C) Cells that contain a transgene for overexpression of GFP-CdaA under the cadmium-dependent MTT1 promoter at time = 0 (A) and after 3 (B) and 6 h (C) of exposure to 2.5 µg/ml cadmium chloride. The cells shown were labeled with anti-GFP (green) and anti-centrin (red) antibodies and DAPI (blue). After 3 h (B), cells accumulate in cell division (almost all cells have an oral primordium). (C) Cells that overproduce GFP-CdaA for 6 h become elongated and have two oral apparatuses near the anterior cell end. (D–G) Examples of individual overproducing cells at 3 h (D–F) or 6 h (G). ma, macronucleus; mi, micronucleus; noa, new oral apparatus (oral primordium); oa, oral apparatus.