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. 2020 Jul 6;219(9):e202002077. doi: 10.1083/jcb.202002077

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© 2020 Jiang et al.

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Figure S2. — TTHERM_00332170 encodes CdaA, a protein with a cyclin E domain that may form a complex with a CDK. (A) Sanger sequencing data of the PCR products obtained with primers that amplified a portion of the TTHERM_00332170/CYC8 coding region containing the candidate causal mutation (shaded). The top panel shows the corresponding sequence of the untransformed cdaA-1 mutant. The bottom panel shows the sequence amplified from one rescue clone. (B) Sequence alignments of cyclins (top) and CDKs (bottom) of H. sapiens and T. thermophila. Residues within 3.5 Å of the cyclin/CDK–binding interface are highlighted in green. Secondary structure information is based on a crystal structure of the human cyclin E1–CDK2 complex (PDB accession no. 1W98; Honda et al., 2005). The red triangles mark the amino acids mutated in the cdaA-1 and cdaA-4 proteins.