Figure S3.

CdaA-GFP localization in conjugating cells and vegetatively growing cells. (A) CdaA-GFP localizes to the junction of conjugating cells. The images show two sides of the same pair formed by a CdaA-GFP–expressing cell and a wild-type cell 5 h after induction of conjugation. The pair was stained with the anti-GFP (red), anti-centrin (green) antibodies and DAPI (blue). CdaA-GFP appears to be present on both the transgenic and nontransgenic side, indicating that CdaA exchanges between the mating partners. (B) TIRF imaging confirms that CdaA-GFP is present in the cell cortex in vivo. Dividing cells expressing CdaA-GFP were partially immobilized and imaged by TIRF microscopy (Jiang et al., 2015). Pairs of phase contrast and fluorescence images are shown for cells whose stages are prior or at the time of cortical subdivision before cytokinesis (a and a′), at the onset of cytokinesis (b and b′), and in advanced cytokinesis (c and c′). Scale bar, 10 μm.