Skip to main content
. 2020 Sep 3;20(5):45. doi: 10.3892/etm.2020.9173

Figure 2.

Figure 2

Non-cytotoxic doses of shikonin inhibit LPS-induced TNF-α expression in RAW 264.7 mouse macrophages. RAW 264.7 cells were treated with 10-250 ng/ml of LPS and/or the indicated doses of shikonin. (A) Contents of TNF-α in culture medium were examined using ELISA assay after 24 h of LPS treatment and shikonin. (B) After 8 h of LPS and shikonin treatment, the relative mRNA expression levels of TNF-α were detected using RT-qPCR. (C) Contents of TNF-α in culture medium were examined using ELISA assay after 24 h of varying LPS treatment and shikonin. (D) Relative mRNA expression levels of TNF-α were assessed using RT-qPCR. *P<0.05 vs. LPS alone group. Reverse transcription-quantitative PCR, RT-qPCR; C, control; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor α.