FIGURE 3.

Overexpression of RIP3 suppresses prostate cancer cells growth in vitro and in vivo. (A) MTS assay was performed to exam the proliferative ability. Upregulated RIP3 caused tumor cells to present with lower proliferative abilities than the negative control and blank control in PC3 and 22RV1 cells. Data shows average of 3 independent experiments ± SEM. Nude mice were injected with lentiviral-delivered RIP3 and control plasmid (NC)-infected PC3 cells. The weight of mice was showed in (B). The tumor growth was measured intermittently and were depicted in the line chart (C). On day 28 after injection of transfected PC3 cells, the tumors were collected for imaging (D,E). Final volume and weight of the tumor were showed in (F,G). The in vivo data was repeated twice, each time with 5 animals per group. All the values represent the means ± SEM.