Skip to main content
. 2020 Aug 26;10:1720. doi: 10.3389/fonc.2020.01720

FIGURE 5.

FIGURE 5

Overexpression of RIP3 induces cell cycle G2 arrest. (A,C) Cell cycle of PC3 and 22RV1 cells were analyzed by flow cytometry. RIP3 transfected groups showed a remarkable increase at the G2 phase compared with blank and NC groups. (B,D) Expression levels of cyclin A2, cyclin B1, CDK1/CDC2, and CDK2 in PC3 and 22RV1 cells were determined by Western blotting, using GAPDH as a control. All tests were repeated three times. Data shows average of 3 independent experiments ± SEM.