FIGURE 6.

RIP3-mediated prostate cancer proliferation inhibition is via necroptosis but not via apoptosis. (A–F) Expression levels of Bax, Bcl-2, Caspase8, Caspase 9,MLKL, and p-MLKL in PC3, 22RV1 cells and mouse tumor tissues were determined by Western blotting, using GAPDH as a control. (G) Morphology was analyzed by microscopy. Arrowheads show cells with cytoplasmic swelling. Representative images were captured at a magnification of 80x (H) LDH release assay proved that the number of cell death in high-expression RIP3 groups were more than negative control. (I) PC3-RIP3 and 22RV1-RIP3 were treated with 5 μM NSA when the cells were seeded in 96-well plates, proliferation inhibition caused by high expression of RIP3 disappeared. Data are mean ± SEM of three replicates.