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. 2020 Sep 9;122(7):151622. doi: 10.1016/j.acthis.2020.151622

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Fig. 2 — Immunoperoxidase and confocal immunofluorescence reactions with Langerin/DC 207 in dolphin (Stenella coeruleoalba) lymph nodes. The lymph node parenchyma capsule (c); the projections of the collagen septa that infiltrate the central portion of the lymph nodes dividing the parenchyma into the lymphoid follicles (If) in the cortex; the medulla (m) shows few immune cells (*) separated by sinusoids (black arrow) and trabeculae (t). Mallory's trichome stain (A). Alveolar macrophages (white arrowheads) and lymphocytic cords (white asterisks) are evident in the lymphoid follicles. Mallory's trichome stain (B). Blood vessels (bv) and granular eosinophils (black arrowheads). Hematoxylin/Eosin staining (C). Langerin/CD207 positive dendritic cells (black arrows and insert). Immunoperoxidase method (D). Langerin/CD207 positive dendritic cells (black arrow) in close contact with alveolar macrophages (white arrowhead). Immunoperoxidase method (E). Langerin/CD207 positive dendritic cells (red arrows) in the cortical region of the lymph nodes. Immunofluorescence method (G-H). Control experiments were conducted excluding the primary antibody (F). Magnifications: A 20x; B-C-d-E-F 100x, G–H 40 × . Scale bars: 3A (50 μm), 3B-C-d-E-F-G-H (20 μm). Reproduction from Lauriano et al., 2020. ELSEVIER LICENSE N° 4881300568867 (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).