Figure 10.

The effect of the loss of Crb1 on the translocation of arrestin3a. A, Diagram represents the method of using the ratios of arrestin3a immunostaining intensities to evaluate the translocation of arrestin3a between the inner segments (IS) and the outer segments (OS). Green dots and blue dots represent the locations used to measure arrestin3a intensities, namely, at the centers of the IS and OS, respectively. B, C, Examples of arrestin3a distribution patterns between the IS and the OS of double cones in 6 dpf WT (B) and crb1 mutant retina (C) in dark, 30 min of light exposure, and 4 h of light exposure. Arrestin3a (red) was visualized with the Zpr1 antibodies, with counterstaining of actin (green) and DAPI (blue) as well as DIC images. Arrows indicate ellipsoids. Scale bar, 20 μm. D, Quantifications and comparison of arrestin3a intensity ratios between WT and crb1 mutant fish; 28 cells from 3 fish were quantified for each group. p values were generated by Student's t test, two-tailed hypothesis. E, Densities of arrestin3a-positive cones in WT and crb1 mutant retina at 6 dpf. Eighteen sections from 9 WT fish and 11 sections from 9 crb1 mutant fish were used for quantification. p values were generated by Student's t test, two-tailed hypothesis.