Figure 8.

The retina of UV light-treated WT and crb1 adult fish displayed condensing/condensed chromatin in UV and blue cone nuclei. A, DAPI staining of radial sections of dorsal retina. Green arrowheads and green arrows indicate condensing UV and RGB cone nuclei, respectively. Yellow arrowheads indicate condensed elliptic nuclei. Red arrowheads indicate more condensed round nuclei. hpl, hours post a 2 h UV light irradiation. Scale bar, 15 μm. B, Comparisons between the ratios of radial to transverse diameters of condensed/condensing cone nuclei in WT and crb1 mutant fish after UV light irradiation. Data are mean ± SEM; 9 sections from 6 individuals were quantified for each group. More than 100 light-damaged cells were quantified at each time point in each group. Data are mean ± SEM. p values were generated by Student's t test, two-tailed hypothesis. C, The inferred morphologic changes and translocation of damaged cone nuclei in the RGB cone layer after UV light irradiation. The top side of images faces the retinal pigment epithelium. The bottom side of images faces the OLM. D, DAPI staining of transverse sections of the dorsal retina of UV light-treated fish. White arrows G, R, and B indicate green, red, and blue cone nuclei, respectively. Green arrows indicate condensing nuclei. Red arrowheads indicate condensed nuclei. Scale bar, 15 μm.