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. 2020 Sep 9;40(37):7119–7132. doi: 10.1523/JNEUROSCI.1121-20.2020

Figure 3.

Figure 3.

The BLAp and PVTp primarily innervate secondary dendrites of NAcSh MSNs. A, Left, Example images of the BLA and PVT where SynaptoTag EGFP and mScarlet were injected, respectively. Right, Example image showing the NAc where the EGFP- and mScarlet-tagged BLAp and mPFCp converged, and MSNs were backfilled by CF633. B, Example z-stacked images showing primary, secondary, and tertiary dendrites with presynaptic terminals from the PVTp (mScarlet) and BLAp (EGFP). C, Images from 0.2-µm-thick sections showing putative presynaptic innervation of MSN dendritic spines by PVTp (mScarlet, left) and BLAp (EGFP, right). Insets, Stacked images (∼5 µm) from the z-axis showing the spines and the putatively presynaptic terminals were within the same plane. D–F, Summaries showing low rates of spines from primary (D), secondary (E), and tertiary (F) dendrites putatively innervated by SynaptoTag-labeled presynaptic terminals from both the BLAp and PVTp. G, H, Summaries showing that both the BLAp and mPFCp preferentially synapsed on secondary dendrites of NAcSh MSNs. *p < 0.05; **p < 0.01.