Table 1.
Calibration levels and quantification parameters (IS, polarity, parent ion m/z, and quantifier ions) for the substances of interest. The calibration ranges are in adequacy with the legal thresholds and the therapeutic ranges.
| Calibration levels (ng/ml) | Quantification parameters | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Level 1 | Level 2 | Level 3 | Level 4 | Level 5 | Polarity | Parent Ion (m/z) → quantifier ion | Qualifier ion for data processing (m/z) | IS | IS concentration (ng/ml) | IS parent ion → quantifier ion | |
| THC | 1 | 2 | 5 | 10 | 20 | + | 315.2319 → 193.1222 | 123.0440 | THC-D3 | 10 | 318.2507 → 196.1413 |
| THC-COOH | 5 | 10 | 25 | 50 | 100 | – | 343.1915 → 245.1546 | 191.1068 | THC-COOH-D9 | 100 | 352.2479 → 254.2108 |
| Cocaine | 10 | 20 | 50 | 100 | 200 | + | 304.1543 → 182.1177 | 82.0657 | Cocaine-D3 | 100 | 307.1731 → 185.1364 |
| Cocaethlyene | 50 | 100 | 250 | 500 | 1,000 | + | 318.1699 → 196.1333 | 82.0651 | Cocaine-D3 | 100 | 307.1731 → 185.1364 |
| Benzoylecgonine | 50 | 100 | 250 | 500 | 1,000 | + | 290.1387 → 168.1020 | 105.0338 | Benzoylecgonine-D3 | 100 | 293.1575 → 171.1204 |
| Amphetamine | 10 | 20 | 50 | 100 | 200 | + | 136.1121 → 91.0547 | 119.0857 | Amphetamine-D8 | 100 | 144.1623 → 97.0921 |
| Methamphetamine | 10 | 20 | 50 | 100 | 200 | + | 150.1277 → 91.0547 | 119.0857 | Amphetamine-D8 | 100 | 144.1623 → 97.0921 |
| MDA | 10 | 20 | 50 | 100 | 200 | + | 180.1019 → 133.0648 | 105.0702 | MDMA-D5 | 100 | 199.1489 → 165.0877 |
| MDMA | 10 | 20 | 50 | 100 | 200 | + | 194.1175 → 163.0753 | 135.0441 | MDMA-D5 | 100 | 199.1489 → 165.0877 |
| Methylphenidate | 10 | 20 | 50 | 100 | 200 | + | 234.1488 → 84.0813 | 56.0503 | Methylphenidate-D10 | 100 | 244.2116 → 93.1376 |
| Morphine | 5 | 50 | 500 | 1,000 | 2,000 | + | 286.1438 → 201.0908 | 229.0858 | Morphine-D3 | 1,000 | 289.1626 → 201.0906 |
| Codeine | 5 | 50 | 500 | 1,000 | 2,000 | + | 300.1594 → 215.1061 | 58.0659 | Codeine-D3 | 1,000 | 303.1783 → 215.1061 |
| Methadone | 5 | 50 | 500 | 1,000 | 2,000 | + | 310.2165 → 105.0339 | 219.1167 | Methadone-D3 | 1,000 | 313.2354 → 105.0337 |
| Tramadol | 5 | 50 | 500 | 1,000 | 2,000 | + | 264.1958 → 58.0659 | – | Tramadol-13C-D3 | 1,000 | 269.2287 → 58.0657 |
| O-Desmethyltramadol | 5 | 50 | 500 | 1,000 | 2,000 | + | 250.1801 → 58.0659 | – | O-Desmethyltramadol-D6 | 1,000 | 256.2178 → 64.1033 |
| Diazepam | 100 | 200 | 500 | 1,000 | 2,000 | + | 285.0789 → 154.0417 | 193.0885 | Nordiazepam-D5 | 1,000 | 276.0947 → 140.0258 |
| Nordiazepam | 100 | 200 | 500 | 1,000 | 2,000 | + | 271.0633 → 140.0262 | 165.0212 | Nordiazepam-D5 | 1,000 | 276.0947 → 140.0258 |
| Oxazepam | 100 | 200 | 500 | 1,000 | 2,000 | + | 287.0582 → 241.0527 | 104.0498 | Nordiazepam-D5 | 1,000 | 276.0947 → 140.0258 |
| Lorazepam | 20 | 50 | 100 | 150 | 300 | + | 321.0192 → 229.0527 | 163.0055 | Alprazolam-D5 | 100 | 314.1215 → 286.1018 |
| Bromazepam | 20 | 50 | 100 | 150 | 300 | + | 316.0080 → 182.0839 | 209.0945 | Alprazolam-D5 | 100 | 314.1215 → 286.1018 |
| Alprazolam | 5 | 10 | 25 | 50 | 100 | + | 309.0902 → 281.0707 | 274.1208 | Alprazolam-D5 | 100 | 314.1215 → 286.1018 |
| Zolpidem | 40 | 100 | 200 | 300 | 600 | + | 308.1757 → 235.1230 | 263.1175 | Zolpidem-D6 | 100 | 314.2134 → 235.1224 |