Table 2.
Results for trueness, precision, and linearity (k is the number of concentration levels, n the number of repetitions by levels, and p the number of non-consecutive days).
| Trueness (%) (k = 4; n = 4; p = 3) | ||||
|---|---|---|---|---|
| Calibration level (ng/ml) | Level 1 | Level 2 | Level 4 | Level 5 |
| THC | 107.3 | 98.4 | 101.8 | 106.2 |
| THC-COOH | 100.9 | 104.2 | 102.4 | 101.9 |
| Cocaine | 101.4 | 102.2 | 103.0 | 103.3 |
| Cocaethlyene | 102.1 | 106.7 | 101.1 | 99.4 |
| Benzoylecgonine | 101.8 | 103.6 | 100.6 | 99.8 |
| Amphetamine | 107.7 | 104.8 | 101.5 | 100.7 |
| Methamphetamine | 102.9 | 101.2 | 100.6 | 97.2 |
| MDA | 104.4 | 105.3 | 103.4 | 98.2 |
| MDMA | 107.2 | 102.2 | 97.6 | 98.3 |
| Methylephenidate | 104.8 | 103.4 | 102.4 | 100.6 |
| Morphine | 101.6 | 96.1 | 100.8 | 100.6 |
| Codeine | 103.5 | 107.6 | 103.2 | 102.4 |
| Methadone | 107.6 | 103.5 | 104.4 | 100.5 |
| Tramadol | 103.8 | 98.5 | 100.4 | 99.6 |
| O-Desmethyltramadol | 98.9 | 100.5 | 98.1 | 99.5 |
| Diazepam | 97.6 | 101.7 | 103.2 | 96.7 |
| Nordazepam | 98.4 | 102.6 | 103.0 | 99.6 |
| Oxazepam | 98.7 | 98.1 | 100.0 | 97.0 |
| Lorazepam | 101.0 | 99.5 | 99.3 | 102.2 |
| Bromazepam | 95.3 | 101.1 | 97.1 | 100.3 |
| Alprazolam | 100.9 | 105.1 | 101.8 | 97.2 |
| Repeatability/intermediate precision (RSD %) (k = 4, n = 4, p = 3) | ||||
| Calibration level (ng/ml) | Level 1 | Level 2 | Level 4 | Level 5 |
| THC | 5.6/5.6 | 3.1/3.9 | 3.9/4.0 | 3.2/7.1 |
| THC-COOH | 7.4/8.3 | 7.2/7.2 | 4.5/4.5 | 5.7/5.7 |
| Cocaine | 7.8/7.8 | 4.0/7.0 | 7.0/7.0 | 3.6/6.3 |
| Cocaethlyene | 3.6/5.7 | 4.7/5.0 | 2.2/2.3 | 4.5/4.5 |
| Benzoylecgonine | 5.0/5.0 | 2.4/3.3 | 2.2/2.2 | 3.1/3.5 |
| Amphetamine | 7.9/7.9 | 5.6/7.6 | 4.7/6.5 | 5.5/5.5 |
| Methamphetamine | 6.7/7.0 | 7.1/7.1 | 4.0/5.7 | 5.5/5.5 |
| MDA | 4.6/5.6 | 4.8/7.7 | 3.0/3.0 | 3.7/3.7 |
| MDMA | 5.2/6.9 | 6.8/6.8 | 4.8/4.8 | 6.4/4.7 |
| Methylephenidate | 3.6/4.1 | 4.1/5.6 | 3.1/3.7 | 3.8/3.8 |
| Morphine | 3.9/7.9 | 3.7/3.7 | 2.2/2.2 | 1.1/1.5 |
| Codeine | 7.2/8.4 | 5.3/6.3 | 5.0/6.2 | 2.8/4.8 |
| Methadone | 8.3/8.3 | 5.3/5.3 | 7.7/7.7 | 4.4/4.4 |
| Tramadol | 3.6/3.6 | 6.5/6.5 | 5.9/5.9 | 3.1/3.1 |
| O-Desmethyltramadol | 6.4/6.9 | 4.0/4.0 | 5.6/5.6 | 3.3/3.3 |
| Diazepam | 11.2/11.2 | 4.6/5.4 | 3.9/4.2 | 5.3/5.3 |
| Nordazepam | 3.8/4.2 | 3.1/3.2 | 2.2/2.2 | 1.9/2.2 |
| Oxazepam | 4.3/4.3 | 5.9/5.9 | 6.6/7.4 | 3.0/4.8 |
| Lorazepam | 8.4/11.0 | 5.5/5.5 | 9.4/9.8 | 4.6/6.5 |
| Bromazepam | 11.6/11.6 | 3.7/3.8 | 3.6/3.6 | 2.5/2.5 |
| Alprazolam | 9.0/9.0 | 5.2/5.6 | 7.0/7.0 | 3.8/4.2 |
| Linearity (k = 4, n = 4, p = 3) | ||||
| Range (ng/ml) | Slope | R2 | LOQ (ng/ml) | |
| THC | 1–20 | 1.0623 | 0.9928 | 1 |
| THC-COOH | 5–100 | 1.0188 | 0.9946 | 5 |
| Cocaine | 10–200 | 1.0339 | 0.9933 | 10 |
| Cocaethlyene | 50–1,000 | 0.9905 | 0.9970 | 50 |
| Benzoylecgonine | 50–1,000 | 0.9959 | 0.9980 | 50 |
| Amphetamine | 10–200 | 1.0044 | 0.9937 | 10 |
| Methamphetamine | 10–200 | 0.9711 | 0.9944 | 10 |
| MDA | 10–200 | 0.9802 | 0.9967 | 10 |
| MDMA | 10–200 | 0.9779 | 0.9934 | 10 |
| Methylephenidate | 10–200 | 1.0046 | 0.9972 | 10 |
| Morphine | 5–2,000 | 1.0063 | 0.9995 | 5 |
| Codeine | 5–2,000 | 1.0237 | 0.9962 | 5 |
| Methadone | 5–2,000 | 1.0077 | 0.9951 | 5 |
| Tramadol | 5–2,000 | 0.9966 | 0.9978 | 5 |
| O-Desmethyltramadol | 5–2,000 | 0.9934 | 0.9977 | 5 |
| Diazepam | 5–2,000 | 0.9688 | 0.9936 | 5 |
| Nordazepam | 5–2,000 | 0.9969 | 0.9987 | 5 |
| Oxazepam | 5–2,000 | 0.9707 | 0.9945 | 5 |
| Lorazepam | 20–300 | 0.9921 | 0.9921 | 20 |
| Bromazepam | 20–300 | 1.0032 | 0.9980 | 20 |
| Alprazolam | 5–100 | 0.9697 | 0.9948 | 5 |
| Zolpidem | 40–600 | 1.0019 | 0.9983 | 40 |