Fig. 1.
SARS-CoV-2 RNA can be detected in direct nasopharyngeal swabs with less sensitivity in RNA simples with >30 Ct values. A. RT-qPCR amplification of SARS-CoV-2 RNA (light blue) and direct nasopharyngeal swab samples preserved in PBS (NSS, light red) using Roche, BGI and SunYat-Sen University as commercial diagnosis kits. Statistical analysis was done using a t-test with Welch's correction. Each dot corresponds to individual data points. B. A positive percentage agreement between RNA (reference) and NSS RT-PCR Bars represent the 95 % confidence intervals computed by the hybrid Wilson/Brown method. C. Total positive percentage agreement between RNA (reference) and NSS RT-qPCR results separated as Ct <30 and >30 in RNA amplification. Bars represent the 95 % confidence intervals computed by the hybrid Wilson/Brown method (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).