FIG 1.

Nodulation kinetics of white clover plants inoculated with R. leguminosarum bv. trifolii strain TA1 or its nodD mutant derivatives. Three biological replicates were performed with 10 technical replicates in each. (A) Average number of nodules per plant. (B) Percentage of plants nodulated. Error bars represent ± standard error of the mean (SEM). Asterisks represent significant difference from TA1 ΔnodD1 determined using one-way analysis of variance (ANOVA) performed on data from day 35 with Dunnett’s multiple comparisons post hoc test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). The same statistical analysis was performed separately on data from day 11 for early infection.