TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference(s) |
|---|---|---|
| Rhizobium species | ||
| TA1 | Wild-type strain isolated from sub clover in the 1950s in Tasmania, Australia | (83, 88) |
| TA1 ΔnodD1 | TA1 nodD1 markerless deletion | This study |
| TA1 ΔnodD2 | TA1 nodD2 markerless deletion | This study |
| TA1 ΔnodD1 ΔnodD2 | TA1 double nodD1 nodD2 markerless deletion | This study |
| WSM1325 | Wild-type strain isolated from an unidentified annual Trifolium species on the Greek Cyclades island of Serifos in 1993 | (79, 89) |
| WSM ΔnodD | WSM1325 nodD markerless deletion | This study |
| Escherichia coli | ||
| ST18 | S17 λpir ΔhemA | (90) |
| Plasmids | ||
| pFAJ1700 | Stable RK2-derived cloning vector, Apr Tcr | (52) |
| pJQ200SK | pACYC derivative, oriVp15A, sacB, Gmr | (61) |
| pSDZ | Derivative of pFAJ1700 carrying promoterless lacZ gene with downstream GFP fusion cassette, Tcr | (59) |
| pPROBE-KT | pVS1/p15A replicon containing promoterless gfp, Kmr | (53) |
| pPROBE-GT | pVS1/p15A replicon containing promoterless gfp, Gmr | (53) |
| pSFTPnodAZ | pSDZ containing TA1 369-bp nodA promoter lacZ transcriptional fusion | This study |
| pSFTPnodFZ | pSDZ containing TA1 330-bp nodF promoter lacZ transcriptional fusion | This study |
| pSFTnodD1 | pFAJ1700 containing TA1 nodD1 with a 286-bp promoter region | This study |
| pSFTnodD2 | pFAJ1700 containing TA1 nodD2 with a 292-bp promoter region | This study |
| pSFTnodD1KT | pPROBE-KT containing TA1 nodD1 with a 286-bp promoter region | This study |
| pSFTnodD2KT | pPROBE-KT containing TA1 nodD2 with a 292-bp promoter region | This study |
| pSFWnodD | pFAJ1700 containing WSM1325 nodD with a 294-bp promoter region | This study |
| pPR3 | pPROBE-KT containing a 336-bp region of the nptII promoter upstream of a promoterless gfp | (22, 52) |
| pAMNHGUSA | pFAJ1700 containing a gusA transcriptional fusion to the CC275e nifH promoter | This study |
| pAMNHCELB | pFAJ1700 containing a celB transcriptional fusion to the CC275e nifH promoter | This study |