TABLE 2.

Primers used in this study^a

Purpose	Primer	Sequence (5′–3′)
Construction of DsufR mutant	sufR-up-Fw	CGGAATTCTCTGCATGAGCGCCAAGT (EcoRI)
	sufR-up-Rev	CGGGATCCCGCCGACGTTTTTCACAAC (BamHI)
	sufR-dw-Fw	CGGGATCCGCCACAACGCATCCGCAA (BamHI)
	sufR-dw-Rev	CCCAAGCTTCGGTGTCGAGGAAGATGACG (HindIII)
	sufR-V1	CCGCCGGACTGGAGCATCA
	sufR-V2	ATGGTGACCTTGGAGCCGATGT
Complementation of DsufR mutant	sufR-C-Fw	CGGGATCCGTGGCCGTCGAGCTGGGT (BamHI)
	sufR-C-Rev	GCTCTAGACTTGAGGCGGAGCTTGGT (XbaI)
	sufR-CM-Fw	CGGGATCCTTCCCGACAGCCTCCTCCG (BamHI)
	sufR-CM-Rev	GCTCTAGAACTCCGCCTGCTTCTCCGT (XbaI)
Construction of His-tagged SufR	His-sufR-Fw	CATGCCATGGAAAACGTCGGCGAGGCAC (NcoI)
	His-sufR-Rev	CGGGATCCTTCCTCCCGGCGGTGCTTG (BamHI)
EMSA	sufRp-Fw	GATGGAAGCTCGCGCACG
	sufRp-Rev	CGGCGGCCTGGGTGAGAC
	sufRpAB-Fw	CGCAGGTCAGTTTAGGTATGCC
	sufRpAB-Rev	GGGTCTCCCGTGCCTCGC
Footprinting assay	sufRp-FAM-Fw	GATGGAAGCTCGCGCACG
	sufRp-Rev	CGGCGGCCTGGGTGAGAC
Lux reporter system	CS-sufR-Fw	CCCTCGAGTCGCCGTACCGCTTCACCAG (XhoI)
	CS-sufR-Rev	CGGGATCCCTCCCGTGCCTCGCCGAC (BamHI)
	sufR-P-Fw	GGGTTGTCAGGCCCTGAGGAA
	sufR-P-Rev	CGGGATCCTCGTTGATGCCGCGCTTGG (BamHI)
Serine and alanine substitution of cysteine residues	sufRC178S-up-Fw	AGTGGGCCGGTCGGAGGG
	sufRC178S-dw-Rev	CTCCGCCTCCGGGATGCC
	sufRC182S-up-Fw	GAGTCTTCCCGACAGCCTCCTC
	sufRC182S-dw-Rev	CGCTGCTTCTCCGCCTCCG
	sufRC195S-up-Fw	AGTCTTCCCGACAGCCTCCTC
	sufRC195S-dw-Rev	GCGGATCTGGTGGTAGACGAC
	sufRC223S-up-Fw	TTCCCGACAGCCTCCTCCG
	sufRC223S-dw-Rev	TGCTCCTTCAGGGCGGTGTC
	sufRC178A-up-Fw	AGTGGGCCGGTCGGAGGG
	sufRC178A-dw-Rev	CTCCGCCTCCGGGATGCC
	sufRE198A-up-Fw	AGTCTTCCCGACAGCCTCCTC
	sufRE198A-dw-Rev	GCGGATCTGGTGGTAGACGAC
5′RACE	Oligo(dT) anchor primer	GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTT
	anchor primer	GACCACGCGTATCGATGTCGAC
	SP1sufR	CGGCGGTGCTTGCGGATG
	SP2sufR	GGTCTCCGCCTCGCACAGC
	SP3sufR	GTCAGGGCGAAGACCTTGGC

^aUnderlining indicates restriction enzyme sites.